Polymorphism of HLA class I genes after restriction by endonucleases Eco RI, Eco RV and Hind III
G Lucotte1, C Coulondre, K Y Ngo
1Laboratoire de Génétique Moléculaire du Centre Nationale de Transfusion Sanguine, Paris, France.
Abstract:
After electrophoresis and transfer onto a hybridization membrane, the restriction endonuclease fragments obtained were probed with a cDNA carrying the nucleotide sequence encoding a class I HLA gene. Polymorphism for presence or absence of various Eco RI and Eco RV and Hind III fragments was noted in a panel of unrelated Parisian people: frequent polymorphism was described for 2 Eco RI restriction fragments of sizes 16.5 and 7.7 kb, for 5 Eco RV restriction fragments of sizes 25, 20, 11.5, 7.8 and 4 kb, and for 3 Hind III restriction fragments of sizes 27.5, 23.5 and 4.7 kb. The French population studied is in Hardy-Weinberg equilibrium for the different variants described.
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