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Related Experiment Videos

Lymphocyte membrane potential assessed with fluorescent probes.

T J Rink, C Montecucco, T R Hesketh

    Biochimica Et Biophysica Acta
    |January 1, 1980
    PubMed
    Summary

    Mouse spleen lymphocytes exhibit a membrane potential of approximately -60 mV, with potassium ions playing a key role in their selective permeability. This study utilized fluorescent probes to assess lymphocyte membrane potential and ion selectivity.

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    Area of Science:

    • Immunology
    • Cell Physiology
    • Biophysics

    Background:

    • Assessing lymphocyte membrane potential is crucial for understanding immune cell function.
    • Fluorescent probes offer a method to measure membrane potential changes in lymphocytes.
    • Potassium ion (K+) selectivity of the lymphocyte membrane influences its electrical properties.

    Purpose of the Study:

    • To determine the membrane potential of mouse spleen lymphocytes using fluorescent probes.
    • To investigate the role of potassium ions in establishing lymphocyte membrane potential.
    • To explore potential calcium-activated potassium channels in lymphocytes.

    Main Methods:

    • Utilized the fluorescent probe 3,3'-Dipropylthiadicarbocyanine (diS-C3-(5)) to measure membrane potential.

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  • Employed high potassium (K+) solutions and the ionophore valinomycin to assess membrane ion selectivity.
  • Used a second probe, bis(1,3-diethylthiobarbiturate)-trimethineoxonol, to validate findings and address probe-specific limitations.

    • Main Results:

    • Lymphocyte membrane potential was estimated to be approximately -60 mV, indicating significant K+ selectivity.
    • High K+ concentrations and valinomycin confirmed the K+-selective nature of the lymphocyte membrane.
    • Evidence suggests the presence of a Ca2+-activated K+ channel in the resting lymphocyte membrane.

    Conclusions:

    • Mouse spleen lymphocytes possess a K+-selective membrane potential of approximately -60 mV.
    • The fluorescent probe diS-C3-(5) provides a valid assessment of lymphocyte membrane potential despite some side effects.
    • The findings support the existence of a Ca2+-activated K+ permeability pathway in lymphocytes.