Related Experiment Video

Updated: Aug 19, 2026

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
09:42

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Preparation of anti-melatonin antibodies and antigenic properties of the molecule

B J Lemaitre, L Hartmann

    Journal of Immunological Methods
    |January 1, 1980
    PubMed

    More Related Videos

    Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope08:09

    Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

    Published on: March 24, 2017

    Generation of Monoclonal Antibodies Against Natural Products12:15

    Generation of Monoclonal Antibodies Against Natural Products

    Published on: April 6, 2019

    Related Concept Videos

    Antibody Structure01:10

    Antibody Structure

    Overview
    Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
    The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
    Antibodies consist of four polypeptide chains: two identical heavy...
    Hybridoma Technology01:31

    Hybridoma Technology

    Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
    Hybridoma Selection
    Commonly used fusion techniques — electroporation, polyethylene glycol...
    Antibody Structure and Classes01:25

    Antibody Structure and Classes

    Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
    The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.