Related Experiment Video
Updated: Aug 14, 2026

Rescue of Recombinant Newcastle Disease Virus from cDNA
Published on: October 11, 2013
Interferon induction by viruses. III. Vesicular stomatitis virus: interferon-inducing particle activity requires
Abstract:
We have measured the interferon-inducing particle (i.f.p.) activity of a ts mutant, GII (I), of vesicular stomatitis virus (VSV) and a non-ts revertant, RI (T1026) in "aged" chick embryo cells and mouse (L(Y) cells at 40.5 and 37.5 degrees C, respectively. Our results suggest that a single i.f.p. suffices to induce a quantum yield of interferon and that there are several times more i.f.p. than plaque-forming particles (p.f.p.) in stock preparations of VSV. Furthermore, while virus replication or amplified RNA synthesis is not required for a particle of VSV to induce interferon, there is a requirement for primary transcription. About one-tenth of the genome must remain intact and be transcribed to synthesize an interferon-inducer moiety. (This represents transcription of about two-thirds of the N protein gene.) We conclude that VSV does not contain a pre-formed inducer of interferon and propose a model for its formation. We suggest that there is a cumulative loss of N (and/or NS and L) protein from the ribonucleoprotein complex during primary transcription, leading ultimately to extensive base-pairing between the genome RNA and its complementary transcript. We suggest that the dsRNA thus formed constitutes the interferon inducer moiety of VSV.
Related Concept Videos
Retrovirus Life Cycles
Immune Response Against Viral Pathogens
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
Size and Structure of Viral Genomes
Inhibitors of Viral Protein Synthesis
Inhibitors Of Virion Release
Inhibitors of Virion Maturation and Assembly

