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Studies on Brucella interferon: chromatographic behaviour and purification.

C Bousquet-Ucla, J Wietzerbin, E Falcoff

    Archives of Virology
    |January 1, 1980
    PubMed
    Summary

    Brucella suis infection induces interferon with antiviral properties. Purification revealed this interferon is glycosylated and hydrophobic, similar to viral interferons, and activates specific protein phosphorylation.

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    Area of Science:

    • Immunology
    • Virology
    • Biochemistry

    Background:

    • Interferons (IFNs) are crucial cytokines in the innate immune response against viral infections.
    • Brucella suis, a bacterial pathogen, can also induce interferon production.
    • Understanding the biochemical properties of bacterial-induced IFNs is essential for their therapeutic potential.

    Purpose of the Study:

    • To characterize the biochemical and functional properties of interferon induced by Brucella suis.
    • To investigate the glycosylation, hydrophobicity, and antigenic properties of Brucella interferon.
    • To explore the mechanism of action of Brucella interferon in inducing antiviral states.

    Main Methods:

    • Induction of interferon in mice by Brucella suis infection.
    • Chromatographic purification using Concanavalian A-Sepharose and Phenyl-Sepharose CL-4B.

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  • Affinity chromatography utilizing antigenic properties for purification.
  • In vitro antiviral assays and protein phosphorylation studies.

    • Main Results:

    • Brucella interferon exhibited complete retention on a lectin column, indicating glycosylation.
    • Hydrophobic properties of Brucella interferon were observed on Phenyl-Sepharose, though masked in crude preparations.
    • Affinity chromatography achieved a 60,000-fold purification of Brucella interferon.
    • Brucella interferon induced specific phosphorylation of a 67,000 MW protein in L cells upon stimulation with double-stranded RNA.

    Conclusions:

    • Brucella interferon shares biochemical characteristics (glycosylation, hydrophobicity) with viral interferons.
    • The purification and characterization of Brucella interferon pave the way for its further investigation.
    • Brucella interferon activates antiviral signaling pathways similar to those induced by viral interferons.