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Related Experiment Videos

Lymphocyte traffic through lymph nodes during cell shutdown.

I McConnell, J Hopkins, P Lachmann

    Ciba Foundation Symposium
    |January 1, 1980
    PubMed
    Summary

    Antigen challenge in lymph nodes causes "cell shutdown," reducing lymphocyte output. This process involves complement and prostaglandins, affecting lymphocyte traffic and T cell populations.

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    Area of Science:

    • Immunology
    • Lymphocyte Biology
    • Sheep Models

    Background:

    • Antigenic challenge significantly alters lymphocyte traffic in lymph nodes.
    • A phenomenon termed 'cell shutdown' involves reduced lymphocyte output without decreased lymph flow.
    • Certain antigens can cause a complete disappearance of B lymphocytes during cell shutdown.

    Purpose of the Study:

    • To investigate the mechanisms underlying cell shutdown in lymph nodes.
    • To explore the role of complement and prostaglandins in lymphocyte traffic modulation.
    • To understand the impact of repeated antigenic stimulation on antigen-specific lymphocyte populations.

    Main Methods:

    • Sheep lymph node studies involving antigenic challenge.
    • Monitoring lymphocyte output and lymph flow in efferent lymph.
    • Induction of localized complement activation in lymph nodes.
    • Analysis of prostaglandin release, particularly PGE2.

    Main Results:

    • Cell shutdown, a reduction in lymphocyte output, was observed following antigenic challenge.
    • Localized complement activation in lymph nodes reproduced cell shutdown and induced prostaglandin release (PGE2).
    • Repeated antigen stimulation promoted selective entry of antigen-specific T lymphocytes into the node, leading to a net loss of reactive T cells from the animal.

    Conclusions:

    • Cell shutdown appears to be a two-step process involving complement activation and prostaglandin release.
    • Antigen-stimulated lymph nodes can selectively trap antigen-specific T lymphocytes.
    • These findings provide insights into lymphocyte recirculation dynamics in response to antigenic stimuli.

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