Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Analysis of plasma urea by continuous flow method using enzymes].

P Lustenberger, J L Orsonneau, G Cornu

    Annales De Biologie Clinique
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Ambulatory high-dose methotrexate administration as central nervous system prophylaxis in patients with aggressive lymphoma.

    Annals of hematology·2021
    Same author

    SuperCam Calibration Targets: Design and Development.

    Space science reviews·2020
    Same author

    Collateral damage: Hidden impact of the COVID-19 pandemic on the out-of-hospital cardiac arrest system-of-care.

    Resuscitation·2020
    Same author

    Radioprotective effects of induced astronaut torpor and advanced propulsion systems during deep space travel.

    Life sciences in space research·2020
    Same author

    Recurrent laryngeal nerve monitoring in children using cricothyroid membrane needle electrodes.

    European annals of otorhinolaryngology, head and neck diseases·2020
    Same author

    Étude comparée de trois souches du coronavirus de la gastroentérite transmissible: Conditions de la réplicationvirale et de la synthèse des antigènes structuraux.

    Annales de l'Institut Pasteur. Virology·2020

    This study presents an automated continuous flow method for measuring plasmatic urea. The new assay, using urease and glutamic-dehydrogenase, offers precise and accurate urea quantification, outperforming traditional chemical methods.

    Area of Science:

    • Clinical Chemistry
    • Biochemical Assays
    • Analytical Chemistry

    Background:

    • Accurate measurement of plasmatic urea is crucial for diagnosing kidney function and metabolic disorders.
    • Existing methods for urea assay can be time-consuming and may lack the precision required for high-throughput analysis.

    Purpose of the Study:

    • To develop and validate an automated continuous flow method for the precise and accurate determination of plasmatic urea.
    • To compare the performance of the new method with a conventional diacetyl-monoxime method.
    • To evaluate the cost-effectiveness and potential interferences of the automated assay.

    Main Methods:

    • Urea hydrolysis by urease enzyme.
    • Ammonium ion (NH+4) quantification using glutamic-dehydrogenase.

    Related Experiment Videos

  • Automated continuous flow system implementation.
  • Comparison with the diacetyl-monoxime colorimetric method.

    • Main Results:

    • The automated continuous flow method demonstrated high precision and accuracy for plasmatic urea assay.
    • Comparison revealed comparable or superior performance to the diacetyl-monoxime method.
    • Analysis of endogenous ammonium ion interference and cost-effectiveness was performed.

    Conclusions:

    • The developed automated continuous flow method provides a reliable and efficient alternative for plasmatic urea determination.
    • This method holds potential for routine clinical laboratory use due to its accuracy, precision, and cost-efficiency.