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Microbial transformation of kepone.

S A Orndorff, R R Colwell

    Applied and Environmental Microbiology
    |February 1, 1980
    PubMed
    Summary

    Pseudomonas aeruginosa strain KO3 and a mixed culture aerobically transform the insecticide Kepone into monohydro-Kepone. This microbial degradation offers a potential pathway for bioremediation of Kepone contamination.

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    Area of Science:

    • Environmental microbiology
    • Bioremediation
    • Chemical ecology

    Background:

    • Kepone (decachlorooctahydro-1,8,8-methano-1H-cyclobuta[cd]pentalen-1-yl) is a persistent organochlorine insecticide.
    • Contamination of water bodies with Kepone poses significant environmental and health risks.
    • Microbial degradation is a promising strategy for the remediation of persistent organic pollutants.

    Purpose of the Study:

    • To investigate the microbial transformation of Kepone by Pseudomonas aeruginosa strain KO3 and a mixed aerobic enrichment culture.
    • To identify the transformation products and quantify their yields.
    • To compare the degradation efficiency of pure and mixed cultures with environmental microorganisms.

    Main Methods:

    • Isolation and culturing of bacteria from sewage sludge lagoon water.
    • Aerobic incubation of Kepone with microbial cultures.
    • Identification of transformation products using gas chromatography (GC) and electron impact mass spectrometry (EIMS).
    • Quantification of dihydro-Kepone using cochromatography with authentic standards.

    Main Results:

    • Pseudomonas aeruginosa strain KO3 and a mixed aerobic culture transformed Kepone into monohydro-Kepone.
    • P. aeruginosa strain KO3 yielded approximately 16% dihydro-Kepone, while the mixed culture yielded about 4%.
    • James River sediment microorganisms produced lower amounts of monohydro-Kepone compared to the pure and mixed cultures.
    • Kepone was not utilized as a sole carbon or energy source by any of the tested bacteria or mixed cultures.

    Conclusions:

    • Pseudomonas aeruginosa strain KO3 and mixed aerobic cultures demonstrate the capability to transform the insecticide Kepone.
    • Microbial transformation yields monohydro-Kepone, suggesting a potential route for Kepone biodegradation.
    • Further research into optimizing these microbial systems could lead to effective bioremediation strategies for Kepone-contaminated environments.

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