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Related Experiment Videos

Nucleolar activity in differentiated cells after stimulation.

F E Arrighi, Y F Lau, A Spallone

    Cytogenetics and Cell Genetics
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

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    Nucleolar organizer region (NOR) activity initiates rapidly in differentiated cells upon stimulation. Silver staining revealed increased NOR activity within hours in activated lymphocytes and fused erythrocytes, correlating with nuclear growth.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Genetics

    Background:

    • Nucleolar organizer regions (NORs) are crucial for ribosome biogenesis and are associated with cell proliferation.
    • Differentiated cells often exhibit suppressed NOR activity, which can be reactivated upon stimulation.
    • Understanding NOR activity dynamics is key to comprehending cellular responses to external stimuli.

    Purpose of the Study:

    • To investigate the temporal dynamics of nucleolar organizer region (NOR) activity initiation in differentiated cells following activation or stimulation.
    • To compare NOR activity changes in different cell types and activation methods.
    • To correlate NOR activity with nuclear size changes in stimulated cells.

    Main Methods:

    • Utilized the silver staining technique to visualize and quantify NOR activity.

    Related Experiment Videos

  • Stimulated human lymphocytes using phytohemagglutinin (PHA).
  • Induced cell-cell fusion between chick erythrocytes and squirrel monkey cells.

    • Main Results:

    • NOR activity was observed as early as 4 hours post-PHA treatment in human lymphocytes.
    • Increased NOR activity in chick erythrocytes was detected between 10 and 22 hours after cell fusion.
    • A positive correlation was found between increasing dormant cell nucleus size and silver-stained area, approaching levels seen in cycling cells.

    Conclusions:

    • NOR activity can be rapidly initiated in differentiated cells upon appropriate stimulation.
    • The timing of NOR reactivation varies depending on the cell type and activation stimulus.
    • Nuclear enlargement is associated with the restoration of NOR activity in stimulated differentiated cells.