Related Experiment Videos
Murine lymphocyte alloantigens. I. The Ly-6 locus
Journal of Immunology (Baltimore, Md. : 1950)
|August 1, 1980
Summary
Researchers further defined the Ly-6 locus and its Ly-6.1 and Ly-6.2 specificities, confirming their identity with ALA-1. Ly-6.2 is also found on B cells, not exclusively T cells.
Area of Science:
- Immunogenetics
- Cell Biology
Background:
- The Ly-6 locus and Ly-6.2 specificity were previously identified.
- Further characterization of this locus and its allelic specificities (Ly-6.1 and Ly-6.2) is needed.
Purpose of the Study:
- To further define the Ly-6 locus and its allelic specificities.
- To investigate the relationship between Ly-6 and other loci, specifically ALA-1.
- To characterize the cellular distribution of Ly-6.2.
Main Methods:
- Back-cross studies and analysis of recombinant inbred (RI) lines.
- Generation and use of the Ly-6 congenic strain C3H.B6-Ly-6b.
- Preparation of antisera and testing on segregating F2 generations.
- Absorption analysis using separated T and B lymphocyte populations.
Main Results:
- Evidence suggests Ly-6 is identical to ALA-1.
- Allelism of Ly-6.1 and Ly-6.2 specificities was confirmed.
- Ly-6.2 is present on both T and B cells, with higher expression on T cells and T blast cells.
- Gene(s) in the A strain, not linked to H-2 or Ly-4, control antibody response magnitude.
Conclusions:
- The Ly-6 locus and its allelic specificities are distinct but closely related to ALA-1.
- Ly-6.2 is expressed on both T and B lymphocytes, challenging previous assumptions of exclusive T cell representation.
- Further investigation into the genetic control of antibody responses between Ly-6 congenic strains is warranted.