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Related Experiment Videos

Postmortem sera and cerebrospinal fluid enzymes.

P Schoning, A C Strafuss

    Journal of Forensic Sciences
    |April 1, 1980
    PubMed
    Summary
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    Amylase and lipase enzyme levels in canine serum remained stable postmortem at cooler temperatures, suggesting their utility in diagnosing pancreatitis. Other enzymes showed limited diagnostic value.

    Area of Science:

    • Veterinary Biochemistry
    • Clinical Pathology
    • Postmortem Diagnostics

    Background:

    • Accurate postmortem diagnosis in dogs relies on evaluating biochemical markers.
    • Enzyme stability in biological samples after death is crucial for diagnostic accuracy.
    • Understanding enzyme behavior under varying postmortem conditions informs diagnostic protocols.

    Purpose of the Study:

    • To assess the stability of key serum enzymes (lipase, amylase, alkaline phosphatase, gamma-glutamyltransferase, alanine aminotransferase) in dogs at different postmortem intervals and temperatures.
    • To determine the diagnostic utility of these enzymes for conditions like pancreatitis.
    • To evaluate the influence of postmortem interval and temperature on enzyme levels in canine serum and cerebrospinal fluid.

    Main Methods:

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    • Analysis of antemortem and postmortem canine sera (n=60) for enzyme activity.
    • Examination of cerebrospinal fluid for specific enzyme markers.
    • Incubation of samples at varying postmortem intervals (3, 6, 12, 24, 48 hours) and temperatures (4°C, 20°C, 37°C).

    Main Results:

    • Canine amylase levels demonstrated stability at 4°C and 20°C, indicating potential diagnostic value for pancreatitis.
    • Lipase levels showed potential as an adjunct diagnostic marker when evaluated with amylase.
    • Serum alkaline phosphatase increased with postmortem interval and was significantly higher at 37°C compared to 4°C.
    • Alanine aminotransferase and gamma-glutamyltransferase showed limited diagnostic utility.

    Conclusions:

    • Amylase and lipase are the most promising enzymes for postmortem diagnosis of canine pancreatitis, particularly when samples are stored at cooler temperatures.
    • Serum alkaline phosphatase levels are temperature- and time-dependent postmortem, limiting their use as a standalone diagnostic marker.
    • Most evaluated enzymes have limited value for postmortem diagnosis in dogs due to instability or lack of significant changes.