Apparent destruction of bovine blood group determinants by chymotrypsin

Although chymotrypsin treatment eliminated the reactivity of many antigenic determinants on the bovine red cell, haemolytic inhibition tests, double diffusion in agar tests, and immunizations provided no conclusive evidence of any of the affected determinants in the soluble digest. Chymotryptic digestion unmasked an apparent cryptantigen, rendering Q-positive cells reactive with a previously Q-negative anti-serum.