NSG banding of sequentially QFQ and RFA banded human acrocentric chromosomes

Sequential QFQ, RFA, and NSG techniques have been demonstrated on the same metaphase to facilitate the maximum characterization of human chromosomes with respect to heteromorphic markers. This combination offers an opportunity to compare the results of the RFA and NSG techniques, which are of particular interest as they concern NOR activity. Furthermore, it is useful in studying abnormalities involving acrocentric chromosomes, somatic association, and the regulation of rRNA synthesis.