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Isoamylases in clinically normal dogs.

J E Stickle, W W Carlton, G D Boon

    American Journal of Veterinary Research
    |April 1, 1980
    PubMed
    Summary
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    Canine serum amylase isoenzymes were analyzed, revealing two main groups in healthy dogs. The cathodal isoamylase is dominant, while the anodal form, originating from the pancreas and uterus, is rare in serum.

    Area of Science:

    • Veterinary Biochemistry
    • Enzymology

    Background:

    • Serum amylase isoenzymes are crucial diagnostic markers in veterinary medicine.
    • Understanding canine amylase isoenzyme profiles aids in diagnosing pancreatic and intestinal diseases.

    Purpose of the Study:

    • To characterize the isoenzyme composition of canine serum amylase.
    • To investigate the tissue origins of different amylase isoenzymes in dogs.
    • To evaluate the impact of feeding on serum amylase activity and isoenzyme patterns.

    Main Methods:

    • Electrophoresis on cellulose acetate membranes was used to separate and identify canine serum amylase isoenzymes.
    • A discontinuous buffer system was employed for optimal separation.
    • Amylase activity in various tissues was quantified and isoenzymes identified.

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    Main Results:

    • Two distinct groups of canine serum amylase isoenzymes were identified in clinically normal dogs.
    • The predominant isoamylase in serum was the cathodal form.
    • The anodal isoamylase, found in pancreas and uterus, was rarely detected in serum and contributed minimally to overall activity.
    • The cathodal isoamylase primarily originated from the intestinal tract.
    • Feeding did not significantly alter total serum amylase activity or its isoenzyme composition within 7 hours.

    Conclusions:

    • Canine serum amylase exhibits a distinct isoenzyme profile dominated by a cathodal form, likely of intestinal origin.
    • The anodal isoamylase, associated with pancreatic and uterine tissues, is a minor component in normal canine serum.
    • Serum amylase levels and isoenzyme patterns are relatively stable post-feeding in dogs.