Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A technique for expressing eukaryotic genes in bacteria.

L Guarente, T M Roberts, M Ptashne

    Science (New York, N.Y.)
    |September 19, 1980
    PubMed
    Summary

    New methods enable efficient expression of cloned eukaryotic genes in Escherichia coli without fusion proteins. These simple genetic manipulations utilize complementary DNA clones and readily available enzymes for straightforward gene product synthesis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The financial burden of healthcare-associated infections: a propensity score analysis in an Italian healthcare setting.

    Infection prevention in practice·2025
    Same author

    Not only COVID-19: a systematic review of anti-COVID-19 measures and their effect on healthcare-associated infections.

    The Journal of hospital infection·2024
    Same author

    SirT3 suppresses hypoxia inducible factor 1α and tumor growth by inhibiting mitochondrial ROS production.

    Oncogene·2011
    Same author

    Sirtuins in aging and disease.

    Cold Spring Harbor symposia on quantitative biology·2008
    Same author

    A therapeutic role for sirtuins in diseases of aging?

    Trends in biochemical sciences·2007
    Same author

    Sirtuins and beta-cell function.

    Diabetes, obesity & metabolism·2007

    Area of Science:

    • Molecular Biology
    • Biotechnology
    • Genetics

    Background:

    • Efficient expression of eukaryotic genes in prokaryotic systems like Escherichia coli is crucial for recombinant protein production.
    • Traditional methods often face challenges with gene introns and result in fusion proteins, complicating downstream applications.

    Purpose of the Study:

    • To present novel methods for the efficient expression of cloned eukaryotic genes in Escherichia coli.
    • To enable the synthesis of unfused, functional eukaryotic gene products.

    Main Methods:

    • Utilizing complementary DNA (cDNA) clones, which lack intervening sequences (introns).
    • Employing simple genetic manipulations with specifically described plasmids.
    • Leveraging commercially available enzymes for the process.

    Main Results:

    • Demonstrated efficient expression of cloned eukaryotic genes in Escherichia coli.
    • Successfully synthesized gene products that are not fused to other amino acid sequences.
    • The methods proved to be simple and reproducible.

    Conclusions:

    • The described methods provide a straightforward and efficient approach for producing unfused eukaryotic proteins in E. coli.
    • These techniques are valuable for biotechnology and molecular biology research requiring recombinant protein expression.

    Related Experiment Videos