Activation of streptolysin S-related cellular potential hemolysin by grinding with glass beads

Cellular potential hemolysin, the precursor to extracellular streptolysin S, was activated by grinding with glass beads on a Vortex mixer. Hemolysin activity was markedly enhanced by the addition of ribonucleic acid-core during the grinding. Determinants for optimum results with the beads included small bead size, prewashing of beads with acetic acid, and appropriate bead volume during grinding. The increased sensitivity of this technique over those previously used revealed cellular potential hemolysin in cells grown in the presence of glucose, which inhibits streptolysin S production. The basis for the increased sensitivity of this procedure may be due to the binding of cellular potential hemolysin or ribonucleic acid to the beads.