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Susceptibility of influenza viruses to interferon and to poly(I) . Poly(C) determined by the plaque reduction method
Abstract:
Susceptibility of eight strains of influenza A and B viruses to interferon and to poly(I) . poly(C) were determined by the plaque reduction method. All strains tested were slightly less susceptible than vesicular stomatitis virus (VSV) in an established line of canine kidney (MDCK) cells. The 50% plaque depression doses (PD50) of poly(I) . poly(C) for influenza A and B viruses were as high as 3.0- to 4.5-fold and 6- to 18-fold that for VSV, respectively. The amounts of interferon required to inhibit plaque formation of influenza A and B viruses by 50% were 3.0-6.2 and 7.3-15.2 units/ml, respectively. The ratio of PD50 of poly(I) . poly(C) for each strain of influenza viruses tested to that for VSV in chick embryo cells was almost the same as in MDCK cells. Furthermore, in chick embryo cells, the strains of influenza virus tested were demonstrated to be much more susceptible to poly(I) . poly(C) than both Newcastle disease virus and vaccinia virus. It is suggested that influenza viruses may be relatively susceptible to interferon and to poly(I) . poly(C).
Insights
Influenza viruses show relative susceptibility to interferon and poly(I)·poly(C) antiviral agents. Studies determined that influenza A and B strains were less susceptible than vesicular stomatitis virus but still responded to these treatments.
Area of Science:
- Virology
- Immunology
- Antiviral Research
Background:
- Interferon and poly(I)·poly(C) are known antiviral agents.
- Understanding the susceptibility of different viruses to these agents is crucial for developing effective treatments.
Purpose of the Study:
- To determine the susceptibility of influenza A and B virus strains to interferon and poly(I)·poly(C).
- To compare the susceptibility of influenza viruses to that of other reference viruses like vesicular stomatitis virus (VSV).
Main Methods:
- Plaque reduction method was used to assess viral susceptibility.
- Experiments were conducted using canine kidney (MDCK) cells and chick embryo cells.
- The 50% plaque depression doses (PD50) for poly(I)·poly(C) and 50% inhibitory doses for interferon were calculated.
Main Results:
- Influenza A and B viruses exhibited susceptibility to both interferon and poly(I)·poly(C).
- Influenza viruses were generally less susceptible than VSV, but significantly more susceptible than Newcastle disease virus and vaccinia virus in chick embryo cells.
- Susceptibility ratios were consistent across both MDCK and chick embryo cell lines.
Conclusions:
- Influenza viruses demonstrate a notable susceptibility to interferon and poly(I)·poly(C).
- These findings suggest potential therapeutic applications of interferon and poly(I)·poly(C) against influenza infections.