Related Experiment Videos
[Reaction of human platelets from ACD and ACD-AG blood to hypotonic shock]
The hypotonic shock response (HSR) of human blood platelets, which reflects functional and morphological integrity of platelets and could be an useful in vitro parameter for demonstration of the in vivo viability of platelets destined for transfusion was investigated. Fresh platelet rich plasma (PRP) and platelet concentrates (PC) from ACD- and ACD-AG-blood were studied by measuring the light absorbance changes at 436 nm after lowering the osmolality to 178 mOsm/kg. The reversed reaction following hypotonic shock was calculated from the maximal slope of the return of the baseline and expressed as absorbance units/min, that second to be the most sensitive parameter of the HSR. The maximal slope of the reversal reaction was significantly higher in ACD-PRP than in ACD-AG-PRP. A statistical difference of the HSR between PRP and PC was found in ACD-blood but not in ACD-AG blood. It was pointed to the great biological deviation of the HSR.
The hypotonic shock response (HSR) of human blood platelets, which reflects functional and morphological integrity of platelets and could be an useful in vitro parameter for demonstration of the in vivo viability of platelets destined for transfusion was investigated. Fresh platelet rich plasma (PRP) and platelet concentrates (PC) from ACD- and ACD-AG-blood were studied by measuring the light absorbance changes at 436 nm after lowering the osmolality to 178 mOsm/kg. The reversed reaction following hypotonic shock was calculated from the maximal slope of the return of the baseline and expressed as absorbance units/min, that second to be the most sensitive parameter of the HSR. The maximal slope of the reversal reaction was significantly higher in ACD-PRP than in ACD-AG-PRP. A statistical difference of the HSR between PRP and PC was found in ACD-blood but not in ACD-AG blood. It was pointed to the great biological deviation of the HSR.