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Unique determinants associated with hybridoma proteins expressing a cross-reactive idiotype: frequency among
It is possible to prepare antisera that recognize individual members of a family of hybridoma proteins in which all members of the family express the cross-reactive idiotype (IdCR) characteristic of the A/J anti-Ars response. These hybridoma-specific reagents also recognize a small percentage of the IdCR+ anti-Ars antibodies, which constitute the normal A/J anti-Ars response. Thus, the previously reported sequence heterogeneity of our IdCR+ hybridoma proteins actually represents components of the normal A/J anti-Ars response. Determinants associated with one particular IdCR+ hybridoma protein, 36-71 (Id36-71), were found on approximately 0.01 to 5.0% of the IdCR+ antibodies in all of the A/J hyperimmune sera that were examined. Determinants associated with 2 other IdCR+ hybridoma proteins, 16-46-4-8 (Id16-46) and 44-10 (Id44-10) were also found in almost all A/J immune sera, again at various concentrations. These results indicate that the heritable trait (IdCR) is expressed as a large number of antibody sequences that probably arise from the somatic diversification of a limited number of germ line genes. The pattern of diversification is qualitatively similar among individual mice but varies with regard to the relative proportions of the individual antibody components.
It is possible to prepare antisera that recognize individual members of a family of hybridoma proteins in which all members of the family express the cross-reactive idiotype (IdCR) characteristic of the A/J anti-Ars response. These hybridoma-specific reagents also recognize a small percentage of the IdCR+ anti-Ars antibodies, which constitute the normal A/J anti-Ars response. Thus, the previously reported sequence heterogeneity of our IdCR+ hybridoma proteins actually represents components of the normal A/J anti-Ars response. Determinants associated with one particular IdCR+ hybridoma protein, 36-71 (Id36-71), were found on approximately 0.01 to 5.0% of the IdCR+ antibodies in all of the A/J hyperimmune sera that were examined. Determinants associated with 2 other IdCR+ hybridoma proteins, 16-46-4-8 (Id16-46) and 44-10 (Id44-10) were also found in almost all A/J immune sera, again at various concentrations. These results indicate that the heritable trait (IdCR) is expressed as a large number of antibody sequences that probably arise from the somatic diversification of a limited number of germ line genes. The pattern of diversification is qualitatively similar among individual mice but varies with regard to the relative proportions of the individual antibody components.