Electron dense staining affinities of mouse oxytalan and elastic fibers

The staining affinity of electron dense stains to mouse palatal elastic fibers and periodontal oxytalan fibers was assessed and compared. Tissues were stained routinely with uranyl acetate and lead citrate or with elastic tissue stains including: (1) silver tetraphenylporphine sulfonate, (2) tannic acid technique, and (3) a high iron diamine sequence. Staining results were assessed with an electron microscope. Palatal elastic fibers demonstrated staining affinity to all elastic stain techniques. Oxytalan fibers did not demonstrate affinity to elastic fiber stains used. Based on electron dense stains, elastic and oxytalan fibers were dissimilar, in contrast to results reported utilizing light microscopic stains.