Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid cytofluorometric method for quantitative DNA determination on fixed smears.

G Mazzini, P Giordano, C M Montecucco

    The Histochemical Journal
    |March 1, 1980
    PubMed
    Summary

    Propidium iodide (PI) offers a rapid and sensitive method for cytofluorometric DNA determination in fixed cells. This DNA staining technique provides accurate results comparable to the Feulgen reaction, with enhanced fluorescence and stability.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Morphological and functional analysis of cryopreserved human sperm: comparison of different freezing protocols.

    Cryo letters·2025
    Same author

    The association between duration of remission, fatigue, depression and health-related quality of life in Italian patients with systemic lupus erythematosus.

    Lupus·2019
    Same author

    Evaluation of the influence of social, demographic, environmental, work-related factors and/or lifestyle habits on Raynaud's phenomenon: a case-control study.

    Clinical and experimental medicine·2019
    Same author

    A preliminary study of an alternative method for evaluating skin sensitizing potential of chemicals.

    International journal of cosmetic science·2019
    Same author

    Protective effect of black relative to white race against non-alcoholic fatty liver disease in patients with severe obesity, independent of type 2 diabetes.

    International journal of obesity (2005)·2018
    Same author

    Primary prevention of cardiovascular disease in patients with systemic lupus erythematosus: case series and literature review.

    Lupus·2017

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Analytical Chemistry

    Background:

    • Cytofluorometric determination of DNA is crucial for cell cycle analysis and ploidy assessment.
    • Traditional methods like the Feulgen reaction can be time-consuming and may have limitations in sensitivity or stability.

    Purpose of the Study:

    • To evaluate the efficacy of propidium iodide (PI) as a fluorescent stain for quantitative DNA analysis in fixed cell smears.
    • To compare the performance of PI staining with the established Feulgen reaction.

    Main Methods:

    • Spectrofluorometric analysis to determine optimal excitation and emission wavelengths for PI.
    • Investigation of DNA-PI binding stoichiometry and specificity across various fixation methods.
    • Enzymatic removal of RNA to assess potential interference.

    Related Experiment Videos

  • Comparison of PI-stained samples with those stained using the Feulgen reaction.

    • Main Results:

    • PI demonstrated optimal conditions for excitation and emission, yielding high fluorescence intensity.
    • The DNA-PI bond showed specificity, and enzymatic RNA removal confirmed minimal interference.
    • PI staining results were comparable to the Feulgen reaction.
    • PI staining offered advantages including speed, high quantum yield, reduced inner filter effect, and lower photodecomposition.

    Conclusions:

    • Propidium iodide is a reliable and efficient stain for cytofluorometric DNA determination.
    • The PI method offers significant advantages over the Feulgen reaction in terms of speed, sensitivity, and stability.
    • PI's spectral properties are favorable for multi-parametric analysis in single cells.