Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Studies on the E. coli groNB (nusB) gene which affects bacteriophage lambda N gene function.

C P Georgopoulos, J Swindle, F Keppel

    Molecular & General Genetics : MGG
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Using focus groups to explore caregiver transitions and needs after placement of family members living with dementia in 24-hour care homes.

    Aging & mental health·2018
    Same author

    Report From the American Society of Transplantation Conference on Donor Heart Selection in Adult Cardiac Transplantation in the United States.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2017
    Same author

    Sirolimus conversion after heart transplant: risk factors for acute rejection and predictors of renal function response.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2014
    Same author

    Randomized controlled trial of sirolimus conversion in cardiac transplant recipients with renal insufficiency.

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2012
    Same author

    Proliferation signal inhibitors and post-transplant malignancies in heart transplantation: practical clinical management questions.

    Clinical transplantation·2011
    Same author

    Cancer of the middle third of the esophagus.

    Memoires. Academie de chirurgie (France)·2010

    New Escherichia coli mutants, groNB, block bacteriophage lambda growth. These mutants also exhibit cold sensitivity, indicating a dual role for the groNB gene in bacterial and phage processes.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Bacteriophage Genetics

    Background:

    • Bacteriophage lambda's growth is regulated by specific gene products.
    • Escherichia coli mutants can confer resistance to phage infection.
    • Temperature-sensitive mutations are valuable tools for genetic analysis.

    Purpose of the Study:

    • To isolate and characterize Escherichia coli mutants affecting bacteriophage lambda growth.
    • To investigate the genetic locus responsible for these mutations.
    • To determine the relationship between phage growth inhibition and bacterial phenotypes.

    Main Methods:

    • Isolation of temperature-resistant mutants (groNB) of Escherichia coli.
    • Characterization of mutants using various bacteriophage lambda strains.

    Related Experiment Videos

  • Genetic mapping of the groNB locus on the E. coli chromosome.
  • Complementation studies using lambda transducing phages.

    • Main Results:

    • Isolation of groNB mutants that inhibit bacteriophage lambda growth at the N gene product level.
    • Identification of the groNB locus at 9 minutes on the E. coli genetic map.
    • Co-inheritance of lambda growth inhibition and bacterial cold sensitivity phenotypes, suggesting linkage.
    • Recessive nature of groNB mutations to the wild-type gene, confirmed by complementation.

    Conclusions:

    • The groNB locus in Escherichia coli plays a crucial role in bacteriophage lambda development.
    • groNB mutations confer a dual phenotype: inhibition of phage lambda growth and bacterial cold sensitivity.
    • The groNB gene is essential for both phage propagation and optimal bacterial growth under specific conditions.