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Related Experiment Videos

Plasminogen activation by normal B lymphocytes, a function associated with the cell membrane.

J L Maillard, C Favreau

    Journal of Immunology (Baltimore, Md. : 1950)
    |March 1, 1981
    PubMed
    Summary

    Mouse spleen B cells activate plasminogen into plasmin via lymphocyte plasminogen activator (LPA), a membrane-associated serine protease. T cells do not activate plasminogen and can inhibit B cell activity.

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    Area of Science:

    • Immunology
    • Biochemistry
    • Cell Biology

    Background:

    • Plasminogen activation is crucial for various physiological processes, including tissue remodeling and immune responses.
    • Lymphocyte involvement in plasminogen activation has been suggested but not fully elucidated.
    • Understanding the specific cell types and mechanisms involved is key to deciphering immune system functions.

    Purpose of the Study:

    • To investigate the role of mouse B cells and T cells in plasminogen activation.
    • To characterize the enzyme responsible for plasminogen activation by lymphocytes.
    • To determine the cellular localization and kinetics of lymphocyte plasminogen activator (LPA).

    Main Methods:

    • Co-culturing purified murine B cells and T cells with purified murine plasminogen.

    Related Experiment Videos

  • Measuring plasmin activity using a fibrinolysis assay with 125I-fibrinogen.
  • Characterizing the enzyme's properties using group-specific antiproteases (e.g., DFP, PMSF) and cell physiology modifiers (cycloheximide, dexamethasone).

    • Main Results:

    • Mouse spleen B cells, particularly medium-sized ones, activate plasminogen to plasmin.
    • Lymphocyte plasminogen activator (LPA) is responsible for this activation and appears to be a membrane-associated serine protease.
    • T cells are ineffective in plasminogen activation and can inhibit B cell activity; enzyme renewal is rapid.

    Conclusions:

    • B cells possess a distinct capability for plasminogen activation mediated by a membrane-bound serine protease (LPA).
    • This finding highlights a novel function of B cells in regulating proteolytic activity within the immune microenvironment.
    • The rapid turnover of LPA suggests a dynamic role in cellular processes and potential therapeutic targeting.