Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Serotoninergic development in the postnatal rat brain.

T Hedner, P Lundborg

    Journal of Neural Transmission
    |January 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Long-term maintenance treatment with omeprazole in children with healed erosive oesophagitis: a prospective study.

    Alimentary pharmacology & therapeutics·2011
    Same author

    Your next of kin or your own career? Caring and working among the 50+ of Europe.

    Journal of health economics·2008
    Same author

    Informal and formal care among single-living elderly in Europe.

    Health economics·2007
    Same author

    Review article: Esomeprazole--enhanced bio-availability, specificity for the proton pump and inhibition of acid secretion.

    Alimentary pharmacology & therapeutics·2003
    Same author

    Safety and efficacy of long term esomeprazole therapy in patients with healed erosive oesophagitis.

    Drug safety·2001
    Same author

    Omeprazole for treatment of chronic erosive esophagitis in children: a multicenter study of efficacy, safety, tolerability and dose requirements. International Pediatric Omeprazole Study Group.

    The Journal of pediatrics·2000
    Same journal

    Cerebral excess release of neurotransmitter amino acids subsequent to reduced cerebral glucose metabolism in early-onset dementia of Alzheimer type.

    Journal of neural transmission·1989
    Same journal

    Dopamine in the rabbit retina and striatum: diurnal rhythm and effect of light stimulation.

    Journal of neural transmission·1989
    Same journal

    Different effects of the calcium antagonists nimodipine and flunarizine on dopamine metabolism in the rat brain.

    Journal of neural transmission·1989
    Same journal

    Oral aspartame and plasma phenylalanine: pharmacokinetic difference between rodents and man, and relevance to CNS effects of phenylalanine. Short note.

    Journal of neural transmission·1989
    Same journal

    Intracerebral dialysis monitoring of striatal dopamine release and metabolism in response to L-dopa.

    Journal of neural transmission·1989
    Same journal

    Postmortem- and cryostability of the potassium-evoked release of [3H]5-hydroxytryptamine from rat cerebral cortical miniprisms.

    Journal of neural transmission·1989
    See all related articles

    The developing rat brain shows a caudal-to-rostral maturation of the serotonergic system, with tryptophan hydroxylase activity being a key factor. This indicates adult-like nerve impulse flow in brainstem serotonin neurons by 28 days.

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Developmental Biology

    Background:

    • The serotonergic system is crucial for various brain functions.
    • Understanding its developmental trajectory is essential for identifying potential disruptions.
    • Rat models offer insights into mammalian neurodevelopment.

    Purpose of the Study:

    • To biochemically characterize the developing serotonergic system in rat brains from 1 to 28 days postnatal.
    • To investigate the maturation sequence of key serotonergic pathway components.
    • To determine the role of tryptophan hydroxylase in this developmental process.

    Main Methods:

    • Biochemical assays of tryptophan, 5-hydroxytryptamine (5-HT), and 5-hydroxyindoleacetic acid (5-HIAA) levels.
    • In vivo measurement of tryptophan hydroxylase activity using 5-hydroxytryptophan (5-HTP) accumulation.

    Related Experiment Videos

  • Assessment of 5-HT disappearance rate after tryptophan hydroxylase inhibition.
  • Regional brain analysis of developmental changes.

    • Main Results:

    • Tryptophan levels peaked around postnatal days 7-10.
    • 5-HTP accumulation and 5-HT levels increased significantly after the second postnatal week.
    • 5-HIAA levels rose rapidly in the brain and more slowly in the CSF.
    • Maturation proceeded in a caudal-to-rostral direction across brain regions.
    • 5-HT half-life decreased with age, with shorter half-lives in caudal regions in younger animals.

    Conclusions:

    • The serotonergic system in rats matures in a caudal-to-rostral sequence.
    • Tryptophan hydroxylase activity appears to be the rate-limiting step in serotonergic system development.
    • By 28 days, the brainstem serotonergic neurons exhibit adult-like activity.