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Related Experiment Videos

Protease inhibitors in middle ear effusions.

B Carlsson, C Lundberg, K Ohlsson

    The Annals of Otology, Rhinology, and Laryngology
    |January 1, 1981
    PubMed
    Summary

    Middle ear effusions in otitis media contain protease inhibitors like alpha 1-antitrypsin and alpha 1-antichymotrypsin. Concentrations varied compared to plasma, with alpha 2-macroglobulin significantly lower.

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    Area of Science:

    • Biochemistry
    • Otolaryngology
    • Immunology

    Background:

    • Serous otitis media involves middle ear fluid potentially containing granulocytes.
    • Granulocytes can release proteases, necessitating investigation of protease inhibitors in middle ear effusions.

    Purpose of the Study:

    • To immunochemically quantify major serum protease inhibitors in middle ear effusions.
    • To compare concentrations of alpha 1-antitrypsin, alpha 1-antichymotrypsin, and alpha 2-macroglobulin in middle ear fluid versus plasma.

    Main Methods:

    • Immunochemical quantification of alpha 1-antitrypsin, alpha 1-antichymotrypsin, and alpha 2-macroglobulin in middle ear effusions.
    • Comparison of inhibitor concentrations with plasma levels and albumin.

    Main Results:

    • Mean alpha 1-antitrypsin concentration was higher in middle ear effusion; alpha 1-antichymotrypsin was slightly lower than in plasma.
    • Concentrations of alpha 1-antitrypsin and alpha 1-antichymotrypsin were lower than expected relative to albumin in middle ear fluid.
    • High molecular weight alpha 2-macroglobulin was significantly reduced in middle ear effusions compared to plasma; a low molecular weight inhibitor was detected.

    Conclusions:

    • Protease inhibitor levels in middle ear effusions differ from plasma, suggesting complex local regulation.
    • The presence of a locally produced low molecular weight inhibitor indicates potential endogenous defense mechanisms in the respiratory epithelium.

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