A simple method to obtain an in vitro primary immune response to dinitrophenyl coupled to bovine gamma-globulin

This paper describes a simple method of achieving a primary in vitro response to dinitrophenyl coupled to a soluble protein. Mice are carrier-immunized, then irradiated and restored with spleen cells from normal donors. Spleen cells are then cultured in the presence of dinitrophenyl on homologous carrier. The anti-hapten antibody synthesis is dose-dependent, and the highest response is obtained with the lowest dose used in the experiments. The higher level of hapten conjugation reduces the immune response.