Use of nuclear Overhauser effect in the study of peptides and proteins

The nuclear Overhauser effect (NOE) leads to changes in the intensity of signal(s) of a set of nuclei as a function of their respective distances. The use of NOE allows to obtain structural informations on peptides and proteins in solution as well as the study of interactions between small ligands and biomolecules. In this review, aspects of the basic theory of the NOE will be presented and the more recent applications of homonuclear and heteronuclear NOE's in biomolecules will be surveyed. Typical examples will be illustrated and limitations of the method will be discussed.