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Updated: Sep 23, 2026

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
Published on: July 29, 2014
Double-stranded RNA dependent protein kinase (s) in rabbit reticulocyte lysates analogous to kinase from
Abstract:
A double-stranded (ds) RNA-dependent protein kinase(s) from haemin-supplemented rabbit reticulocyte lysates in purified by chromatography on columns of poly(I).poly(C)-Sepharose. This method provides a more than 1000-fold purifications of the kinase(s) in a single step and a high yield. The purified kinase is responsible for the phosphorylation of (a) a polypeptide of molecular weight 67000, (b) the smallest subunit eIF-2 and (c) added histones. These kinase activities are comparable to the dsRNA-dependent protein kinase activities from interferon-treated cells.
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