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[Isolation of a cellular hybrid secreting an antibody specific for human leukocyte interferon]
A cellular hybrid secreting a Mouse monoclonal immunoglobulin (IgG) specific of human leukocyte interferon has been obtained, following fusion of a nonsecreting variant of the myeloma P3X63 Ag8 with splenic lymphocytes from a Mouse immunized against interferon incorporated into liposomes. The antibody also recognizes human interferon made by a bacterial recombinant and a fraction of interferon made by Namalva cells. Mass production of the antibody has been achieved using ascitic growth of the hybrid in Mice.
A cellular hybrid secreting a Mouse monoclonal immunoglobulin (IgG) specific of human leukocyte interferon has been obtained, following fusion of a nonsecreting variant of the myeloma P3X63 Ag8 with splenic lymphocytes from a Mouse immunized against interferon incorporated into liposomes. The antibody also recognizes human interferon made by a bacterial recombinant and a fraction of interferon made by Namalva cells. Mass production of the antibody has been achieved using ascitic growth of the hybrid in Mice.