Related Experiment Videos
Interferon-resistant cell line lacks fatty acid cyclooxygenase activity
Abstract:
A clone of L1210 mouse leukemia cells selected for resistance to both the antiviral and anticellular properties of mouse interferon were essentially devoid of fatty acid cyclooxygenase activity. Experiments in which broken cell preparations were mixed or the two cell types were cultivated together failed to indicate the presence of a diffusible enzyme inhibitor. Fatty acid lipoxygenase activity of similar magnitude was detectable in both cell types. A selective impairment of fatty acid cyclooxygenase in interferon-resistant cells is consistent with recently described data suggesting that this enzyme may play a crucial role in mediating the antiviral and anticellular effects of interferon.
Insights
Mouse interferon resistance in leukemia cells is linked to a lack of fatty acid cyclooxygenase activity. This suggests the enzyme is crucial for interferon
Area of Science:
- Biochemistry
- Cell Biology
- Immunology
Background:
- Mouse interferon exhibits antiviral and anticellular properties.
- L1210 mouse leukemia cells were utilized in this study.
- Interferon resistance mechanisms are not fully understood.
Purpose of the Study:
- To investigate the biochemical basis of interferon resistance in L1210 cells.
- To determine the role of fatty acid cyclooxygenase in interferon's effects.
Main Methods:
- Selection of L1210 cells resistant to mouse interferon.
- Assay of fatty acid cyclooxygenase and lipoxygenase activity in cell preparations.
- Co-cultivation experiments and mixing of broken cell preparations.
Main Results:
- Interferon-resistant L1210 cells showed significantly reduced fatty acid cyclooxygenase activity.
- No evidence of a diffusible enzyme inhibitor was found.
- Fatty acid lipoxygenase activity was comparable in both cell types.
Conclusions:
- A selective impairment of fatty acid cyclooxygenase is associated with interferon resistance.
- Fatty acid cyclooxygenase may be a key mediator of interferon's antiviral and anticellular actions.