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Interferon-resistant cell line lacks fatty acid cyclooxygenase activity
Science (New York, N.Y.)
|April 17, 1981
Summary
Mouse interferon resistance in leukemia cells is linked to a lack of fatty acid cyclooxygenase activity. This suggests the enzyme is crucial for interferon
Area of Science:
- Biochemistry
- Cell Biology
- Immunology
Background:
- Mouse interferon exhibits antiviral and anticellular properties.
- L1210 mouse leukemia cells were utilized in this study.
- Interferon resistance mechanisms are not fully understood.
Purpose of the Study:
- To investigate the biochemical basis of interferon resistance in L1210 cells.
- To determine the role of fatty acid cyclooxygenase in interferon's effects.
Main Methods:
- Selection of L1210 cells resistant to mouse interferon.
- Assay of fatty acid cyclooxygenase and lipoxygenase activity in cell preparations.
- Co-cultivation experiments and mixing of broken cell preparations.
Main Results:
- Interferon-resistant L1210 cells showed significantly reduced fatty acid cyclooxygenase activity.
- No evidence of a diffusible enzyme inhibitor was found.
- Fatty acid lipoxygenase activity was comparable in both cell types.
Conclusions:
- A selective impairment of fatty acid cyclooxygenase is associated with interferon resistance.
- Fatty acid cyclooxygenase may be a key mediator of interferon's antiviral and anticellular actions.