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PAS-positive polymorphonuclear leucocytes in corneal ulcers.

J U Prause, O A Jensen

    Acta Ophthalmologica
    |August 1, 1980
    PubMed
    Summary
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    Polymorphonuclears (PML) in corneal ulcers indicate inflammation. PAS-positive PML suggest glycogen, while PAS-negative PML may indicate higher protease activity, serving as an indicator of ulcer activity.

    Area of Science:

    • Ophthalmology
    • Pathology
    • Cell Biology

    Background:

    • Severe keratitis often presents as corneal ulcers, indicating significant ocular inflammation.
    • Understanding the inflammatory infiltrate, particularly polymorphonuclears (PML), is crucial for assessing corneal ulcer severity.

    Purpose of the Study:

    • To histologically and ultrastructurally examine inflammatory reactions in severe corneal ulcers.
    • To correlate the presence and characteristics of PML with the stage of corneal ulceration.
    • To evaluate the utility of Periodic Acid-Schiff (PAS) staining for assessing PML activity.

    Main Methods:

    • Histological examination of 50 severe keratitis cases with corneal ulcers.
    • Ultrastructural study of 14 similar cases using Transmission Electron Microscopy (TEM).

    Related Experiment Videos

  • Cell counts of inflammatory cells (PML, eosinophils, lymphocytes, plasma cells) and PAS staining for PML glycogen content.

    • Main Results:

    • PML were significantly more frequent in ulcerated areas (32/50 cases).
    • Most PML were PAS-positive, containing glycogen (26/50 cases).
    • PAS-negative PML were associated with increased plasma cells and suggest high protease activity due to degranulated PML.

    Conclusions:

    • PAS stainability of PML can serve as an indicator of activity in corneal ulcers.
    • PAS-negative PML in corneal ulcers may signify increased protease levels and ongoing tissue degradation.
    • Ultrastructural analysis revealed highly phagocytic, partly degranulated PML with reduced glycogen content.