Effect of different preservation solutions and substrates on metabolic viability of kidney tubules

Glucose production, pyruvate uptake and lactate production were taken as metabolic viability tests for isolated rat kidney tubules preserved in hypothermia. The results depend on the type of preservation solution used. Species specific serum is the only solution sustaining cellular metabolism at a normal level. Using Collins solution all viability parameters showed the lowest results. Addition of certain substrates to the Krebs-Henseledt solution improves metabolic viability.