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Stabilization of transcobalamins against heating on sephadex G-50
Abstract:
The human serum stabilized on Sephadex G-50 did not show any changes in the distribution of radioactive vitamin B12 bound to transcobalamins during incubation at 56 degrees C (30 and 120 min.) and 80 degrees C (20 min.), whereas, in the non-stabilized serum, heating increased the vitamin 57CoB12 in transcobalamin I and III fraction and decreased or completely abolished the amount of radioactive vitamin B12 bound to transcobalamin II.