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Related Experiment Videos

Cell surface changes accompanying myoblast differentiation.

L T Furcht, G Wendelschafer-Crabb, P A Woodbridge

    Journal of Supramolecular Structure
    |January 1, 1977
    PubMed
    Summary

    Cell surface receptors on myoblasts change distribution during differentiation, a process influenced by hormones like insulin and dexamethasone. This study investigates these dynamic changes and hormonal effects on myoblast differentiation.

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    Area of Science:

    • Cell Biology
    • Developmental Biology
    • Biochemistry

    Background:

    • Myoblasts are mononucleated cells that fuse to form multinucleated myotubes during differentiation.
    • Understanding cell surface dynamics and hormonal regulation is crucial for studying myoblast differentiation.

    Purpose of the Study:

    • To investigate the dynamic changes in Concanavalin A (Con-A) lectin receptor mobility on myoblasts during differentiation.
    • To examine the role of hormones, specifically insulin and dexamethasone, in modulating myoblast differentiation and proliferation.

    Main Methods:

    • Utilized Concanavalin A (Con-A) lectin to probe cell surface receptor distribution on undifferentiated and differentiated myoblasts at 37°C.
    • Employed glutaraldehyde prefixation to assess the role of cell surface mobility in Con-A receptor redistribution.

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  • Administered insulin and dexamethasone to cultures to evaluate their effects on myoblast differentiation and proliferation.
  • Performed ultrastructural analysis to observe extracellular matrix interactions with Con-A.

    • Main Results:

    • Con-A receptors showed uniform distribution on undifferentiated myoblasts but redistributed into patches, caps, and vesicles on differentiating cells and myotubes.
    • Glutaraldehyde prefixation prevented redistribution, indicating its dependence on cell surface dynamics.
    • Insulin (10 µg/ml) significantly enhanced myoblast differentiation, preceded by proliferation stimulation.
    • Dexamethasone inhibited differentiation; 10 µM stimulated proliferation, while 100 µM suppressed it.
    • An extracellular filamentous matrix binding Con-A was observed, but without redistribution similar to cell membrane receptors.

    Conclusions:

    • Myoblast differentiation involves significant redistribution of Con-A receptors on the cell surface, dependent on membrane dynamics.
    • Insulin and dexamethasone exert differential effects on myoblast differentiation and proliferation, highlighting their complex regulatory roles.
    • The extracellular matrix interacts with Con-A but does not exhibit the same dynamic receptor redistribution seen on the cell membrane.