[Serum proteins of growing Gunn rats. Quantitative analysis with agarose gel electrophoresis (author's transl)]

Microprocesses of quantitative agarose gel electrophoresis and biuret total protein evaluation were used for the quantitative estimation of the serum proteins of homozygous and heterozygous Gunn rats. For the analyses carried out in the course of hyperbilirubinemia, 6 microliter of serum were necessary in each case, of which 50-80 microgram were used for electrophoresis. The quantitative evaluation of the phoretogram curves plotted at two wavelengths was carried out with the aid of an analog computer. Eleven fractions were quantitatively covered by the process. Age-related concentration shifts were observed for a number of fractions, especially for albumin, transferrin, and IgG. The results obtained from a large number of animals in longitudinal and cross-sectional examination corroborate various reports found in the relevant literature.