Victoria blue-nuclear fast red stain for HBs antigen detection in paraffin section

Hepatitis B surface antigen, in paraffin embedded sections, can be stained by victoria blue and recognized easily against the background, including the cytoplasm and nucleus, stained by nuclear fast red. This new staining method has many merits such as easy staining procedure, long stability of dye solution, negative stainability of ordinary bile pigment, etc., and seems to be quite useful for routine as well as research works in hepatology.