Evaluation of the staining conditions of immunofluorescence in primary glomerulonephritis

A study of the evaluation of various staining conditions of immunofluorescence in renal biopsy specimens is described. Renal biopsy specimens were obtained from 52 patients with various types of primary glomerulonephritis, and stained with fluorescein-conjugated anti-human IgG, IgA, or IgM antisera under various staining conditions. The results indicated that IgM was more prominently stained at 4 degrees C overnight than at 37 degrees C or room temperature by the immunofluorescent staining. Both IgG and IgA did not show any differences in the pattern of staining under different conditions. It is suggested that the different staining conditions of immunofluorescence in patients with glomerulonephritis is useful for the evaluation of the classes of immunoglobulins deposited in the glomeruli.