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Updated: Aug 8, 2026

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Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
Cloning of alpha 2u globulin cDNA using a high efficiency technique for the cloning of trace messenger RNAs
Gene
|March 1, 1981
Abstract:
An extremely high-efficiency technique is described for cloning double-stranded (ds) cDNAs in Escherichia coli. The method, which uses two synthetic oligonucleotide linkers rather than one, results in approx. 200--500 recombinant clones per ng of ds cDNA. This technique was used to clone a cDNA comprising 95% of the full length of the mRNA of alpha 2u globulin, a male rat liver protein, which represents approx. 1% of hepatic messenger RNA. The cloned probe was applied to study the complex hormone controls of alpha 2u globulin mRNA in male and female rats.
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Since the...
Since the...

