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Variations in the response of cloned murine friend erythroleukemia cells to different inducers
Abstract:
Cells of the line 3BM-78 derived from murine bone marrow cells infected in vitro with polycythemic Friend leukemia virus (FLV-P) produce virus with spleen focus-forming activity (SFFV) and can be induced to synthesize hemoglobin. Fifteen clones, isolated from this line, have been analyzed in detail for the effect of different inducing agents (dimethyl-sulfoxide, DMSO; hexamethylene bisacetamide, HMBA; and sodium butyrate, SB) on the synthesis of hemoglobin and virus at the clonal level. All the clones proved to be inducible with one or more of the agents, but the degree of the response depended on the type and concentration of the agent used. In general, the effectiveness of the agent--within the usual range of concentration for induction--both for hemoglobin and for virus synthesis, was in the order HMBA greater than DMSO greater than SB. Reverse transcriptase activity was, however, more easily induced than hemoglobin synthesis in that stimulation was seen at lower concentrations of the same inducing agent. This clonal analysis confirmed that virus and hemoglobin production are regulated independently in these erythroleukemic cells chronically infected with FLV-P.
Insights
Murine erythroleukemic cells infected with Friend leukemia virus (FLV-P) can produce hemoglobin and virus. Different agents like HMBA, DMSO, and SB induce these processes independently at the clonal level.
Area of Science:
- * Hematology
- * Virology
- * Molecular Biology
Background:
- * Murine erythroleukemic cells (3BM-78 line) derived from bone marrow infected with polycythemic Friend leukemia virus (FLV-P) exhibit virus production and hemoglobin synthesis potential.
- * These cells are chronically infected with FLV-P, which confers spleen focus-forming activity (SFFV).
Purpose of the Study:
- * To analyze the effect of various inducing agents on hemoglobin and virus synthesis at the clonal level.
- * To determine the relative effectiveness of different inducers and their concentrations.
- * To investigate the independent regulation of virus and hemoglobin production in erythroleukemic cells.
Main Methods:
- * Isolation and detailed analysis of fifteen distinct clones from the 3BM-78 cell line.
- * Treatment of clones with inducing agents: dimethyl sulfoxide (DMSO), hexamethylene bisacetamide (HMBA), and sodium butyrate (SB).
- * Measurement of hemoglobin synthesis and virus production (including reverse transcriptase activity) in response to inducers.
Main Results:
- * All analyzed clones were inducible for hemoglobin and/or virus synthesis by at least one agent.
- * Inducer effectiveness varied by type and concentration, generally following the order HMBA > DMSO > SB for both hemoglobin and virus synthesis.
- * Reverse transcriptase activity was more readily induced than hemoglobin synthesis, showing stimulation at lower inducer concentrations.
- * Clonal analysis confirmed independent regulation of virus and hemoglobin production.
Conclusions:
- * Hemoglobin and virus production are independently regulated processes in FLV-P-infected erythroleukemic cells.
- * The choice and concentration of inducing agents significantly impact the extent of hemoglobin and virus synthesis.
- * HMBA is generally the most effective inducer among those tested, followed by DMSO and then SB.