Nucleic acid fixation to cyanuric chloride-activated paper. Use for nucleic acid transfer and affinity binding

A procedure for the binding of nucleic acids to cyanuric chloride-activated paper is described. The pH value of the binding buffer has a substantial influence on the binding reaction and on the stability of binding. Substantial and stable binding of DNA occurs only in a pH range between 5 and 6. At pH 5.5 up to 20 microgram are bound per cm2 activated paper. Electrophoretically separated DNA and RNA are transferred to cyanuric chloride-activated paper using an electrotransfer method. The hybridization of transferred DNA and RNA to specific sequences is demonstrated.