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Related Experiment Videos

Human lupus inclusions and interferon.

S A Rich

    Science (New York, N.Y.)
    |August 14, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Bromodeoxyuridine-substituted Raji cells secrete a protein that induces lupus inclusions in other cells. This protein, found in conditioned medium, is distinct from viruses and sensitive to pronase treatment, suggesting a novel pathogenic mechanism.

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    Area of Science:

    • Cell Biology
    • Virology
    • Immunology

    Background:

    • Raji cells, a human B lymphoblastoid cell line, are of Burkitt lymphoma origin.
    • Lupus inclusions are abnormal structures observed in certain cellular conditions.

    Purpose of the Study:

    • To investigate the factors responsible for lupus inclusion formation in Raji cells.
    • To identify the nature of the inducing agent secreted by bromodeoxyuridine-substituted Raji cells.

    Main Methods:

    • Culturing Raji cells with DNA thymidine substituted by bromodeoxyuridine.
    • Conditioning the growth medium with these substituted cells.
    • Ultracentrifugation and pronase treatment of the conditioned medium.
    • Assessing lupus inclusion formation in non-substituted Raji cells.

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    Main Results:

    • Bromodeoxyuridine-substituted Raji cells formed lupus inclusions in conditioned medium.
    • Viral removal via ultracentrifugation did not prevent inclusion formation.
    • Pronase treatment abolished the activity of the conditioned medium.
    • Interferon was detected in the conditioned medium, and exogenous interferon also induced inclusion formation.

    Conclusions:

    • A protein secreted by bromodeoxyuridine-substituted Raji cells induces lupus inclusions.
    • This protein's activity is sensitive to enzymatic degradation (pronase) and distinct from viral agents.
    • Interferon may play a role in the induction of lupus inclusions in this cellular model.