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Updated: Aug 12, 2026
08:04
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
Staining of DNA with uranylacetate in hydrolysed ultrathin sections
Acta Histochemica
|January 1, 1981
Abstract:
Rat liver and hepatoma cells fixed with formaldehyde, embedded into Epon and treated on sections with 5 n HCl and then with aqueous uranylacetate show preferential DNase-sensitive reaction. The reaction is highly dependent upon proper fixation, hydrolysis improves its specificity. The binding of the contrast with DNA is of ionic nature. Because of its simplicity, sufficient contrast and resolution the suggested technique is recommended for ultrastructural studies of DNA-containing substrates.
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