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Updated: Oct 13, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
Circulating autoantibodies to hidden mitochondrial antigenic determinants in mammals
Abstract:
Autoantibodies to equine cytochrome c and to porcine malic dehydrogenase are shown in equine and porcine serum, respectively, by immunodiffusion. A confluence of arcs between human plasma and rabbit, horse and pig serum against these antigens suggests the presence of antibodies in human plasma to cytochrome c and malic dehydrogenase. The use of tris buffer containing a low concentration of Dodecyl sodium sulfate for cytochrome c and of glycerol for malic dehydrogenase maintains their solubility and enhances their participation in antibody-antigen immunoprecipitation possibly by unravelling the peptide chains and exposing reactive antigenic determinants hidden within the interstices of the cytochrome c and malic dehydrogenase molecules.
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