Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Feedback controlled nerve fiber impalement.

M H Bennett

    Electroencephalography and Clinical Neurophysiology
    |August 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel device for automatic nerve fiber impalement, significantly improving intracellular recording stability and efficiency in electrophysiology research.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Recommendations for rescue of a submerged unresponsive compressed-gas diver.

    Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·2013
    Same author

    A retrospective audit of three different regional anaesthetic techniques for circumcision in children.

    Anaesthesia and intensive care·2010
    Same author

    Arterial gas embolism: a review of cases at Prince of Wales Hospital, Sydney, 1996 to 2006.

    Anaesthesia and intensive care·2008
    Same author

    Recompression and adjunctive therapy for decompression illness.

    The Cochrane database of systematic reviews·2007
    Same author

    The synthesis and enzymic hydrolysis of (E)-2-[2,3-2H2]propenyl glucosinolate: confirmation of the rearrangement of the thiohydroximate moiety.

    Phytochemistry·2007
    Same author

    Hyperbaric oxygen for idiopathic sudden sensorineural hearing loss and tinnitus.

    The Cochrane database of systematic reviews·2007
    Same journal

    Coming to terms with brain waves.

    Electroencephalography and clinical neurophysiology·2014
    Same journal

    Habituation of lower leg stretch responses in Parkinson's disease.

    Electroencephalography and clinical neurophysiology·2000
    Same journal

    Asymmetry of cortical excitability revealed by transcranial stimulation in a patient with focal motor epilepsy and cortical myoclonus.

    Electroencephalography and clinical neurophysiology·2000
    Same journal

    Evoked isometric muscle contractions in myopathies: analysis of pathophysiological properties by different stimulus patterns.

    Electroencephalography and clinical neurophysiology·2000
    Same journal

    Task-related coherence and task-related spectral power changes during sequential finger movements.

    Electroencephalography and clinical neurophysiology·2000
    Same journal

    Electrophysiological studies in mild idiopathic carpal tunnel syndrome.

    Electroencephalography and clinical neurophysiology·2000
    See all related articles

    Area of Science:

    • Neuroscience
    • Electrophysiology
    • Biomedical Engineering

    Background:

    • Intracellular recording requires precise microelectrode placement.
    • Achieving stable recordings from nerve fibers is technically challenging.
    • Existing methods can be time-consuming and prone to instability.

    Purpose of the Study:

    • To describe a device for automatic impalement of nerve fibers.
    • To enable more stable intracellular recordings from neural tissues.
    • To enhance the efficiency of electrophysiological experiments.

    Main Methods:

    • Utilized cell membrane potential to halt microelectrode advancement.
    • Modified the device to stop microdrive advance upon detecting action potentials.
    • Interconnected commercially available electrophysiology instruments to build the circuit.

    Related Experiment Videos

    Main Results:

    • The device automates the impalement of nerve fibers.
    • Demonstrated applicability for intracellular recording from various cell types.
    • Increased the number of stable cell penetrations by 1-2 orders of magnitude in dorsal column fibers of the cat.

    Conclusions:

    • The developed device significantly improves the efficiency and stability of intracellular recordings.
    • The technique is adaptable for recording from diverse cell types.
    • This innovation offers a valuable tool for electrophysiology research.