Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Purification and characterization of streptococcal proliferative factor.

E O Rasmussen, K D Wuepper

    The Journal of Investigative Dermatology
    |August 1, 1981
    PubMed
    Summary

    Researchers developed an in vivo method to produce and purify streptococcal proliferation factor, a substance linked to Group A Streptococcus infections like scarlet fever and psoriasis flares.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Structure and transcriptional regulation of the human cystatin A gene. The 12-O-tetradecanoylphorbol-13-acetate (TPA) responsive element-2 site (-272 to -278) on cystatin A gene is critical for TPA-dependent regulation.

    The Journal of biological chemistry·1998
    Same author

    Cloning of human keratolinin cDNA: keratolinin is identical with a cysteine proteinase inhibitor, cystatin A, and is regulated by Ca2+, TPA, and cAMP.

    The Journal of investigative dermatology·1997
    Same author

    Gene for familial psoriasis susceptibility mapped to the distal end of human chromosome 17q.

    Science (New York, N.Y.)·1994
    Same author

    Psoriasis vulgaris: a genetic approach.

    The Journal of investigative dermatology·1990
    Same author

    Repeat direct immunofluorescence to discriminate pemphigoid from epidermolysis bullosa acquisita.

    Archives of dermatology·1990
    Same author

    Toxic epidermal necrolysis. Unmuddying the waters.

    Archives of dermatology·1987

    Area of Science:

    • Microbiology
    • Immunology
    • Dermatology

    Background:

    • Group A streptococcal infections are linked to scarlet fever and guttate psoriasis flares.
    • Streptococcal culture filtrates contain a factor that stimulates keratinocyte and lymphocyte proliferation.
    • Previous studies indicated a need for understanding this proliferative factor.

    Purpose of the Study:

    • To outline an in vivo method for producing streptococcal proliferation factor.
    • To purify the factor and characterize its physical properties.
    • To establish a rapid purification process.

    Main Methods:

    • Group A streptococci (Type 12, Strain NY5) were cultured in vivo using dialysis casing in rabbit peritoneum.
    • Streptococcal exoproteins were isolated via centrifugation and millipore filtration.
    • Purification involved differential solubility and molecular sieve chromatography; characterization used SDS-PAGE, ultracentrifugation, and isoelectric focusing.

    Main Results:

    • A rapid, 2-step purification process for streptococcal proliferative factor was established.
    • The factor's relative molecular weight was determined using SDS-PAGE.
    • Sedimentation coefficient (2.7S) and isoelectric point (pI 5.0) were characterized.

    Conclusions:

    • In vivo incubation of streptococci in synthetic media is an effective method for producing streptococcal proliferative factor.
    • The physical properties of the streptococcal proliferative factor have been successfully established.
    • This research provides a foundation for further investigation into the role of this factor in associated diseases.

    Related Experiment Videos