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Related Experiment Videos

Prostate antigen: a new potential marker for prostatic cancer.

M C Wang, L D Papsidero, M Kuriyama

    The Prostate
    |January 1, 1981
    PubMed
    Summary

    Researchers identified a prostate-specific antigen (PSA) in prostate tissues and seminal plasma. Elevated PSA levels in blood indicate prostate cancer, making it a valuable diagnostic marker.

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    Prostate-specific antigen levels in 1695 men without evidence of prostate cancer. Findings of the American Cancer Society National Prostate Cancer Detection Project.

    Cancer·1992

    Area of Science:

    • Biochemistry
    • Immunology
    • Oncology

    Background:

    • Prostate cancer diagnosis relies on various markers.
    • A specific antigen distinct from acid phosphatase is present in prostate tissues and fluids.

    Purpose of the Study:

    • To identify and characterize a novel prostate-specific antigen.
    • To develop a sensitive assay for detecting this antigen in serum.
    • To evaluate its potential as a biomarker for prostate cancer.

    Main Methods:

    • Immunologic procedures for antigen identification in tissues and fluids.
    • Immunoperoxidase staining to determine cellular localization.
    • Purification and characterization of the antigen (molecular weight, isoelectric point).
    • Development of a sandwich-type, peroxidase-linked immunosorbent assay (ELISA).

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    Main Results:

    • A prostate-specific antigen (PSA) was identified and localized to prostatic epithelial cells.
    • Purified PSA has a molecular weight of 33,000-34,000.
    • Serum PSA levels were significantly elevated in prostate cancer patients compared to normal males.
    • The developed ELISA can detect as little as 0.1 ng/mL of PSA.

    Conclusions:

    • Prostate-specific antigen (PSA) is a distinct marker found in prostate tissues and seminal plasma.
    • Elevated serum PSA levels are associated with prostate cancer.
    • PSA is a promising biomarker for the early detection and diagnosis of prostate cancer.