Interferon (type-alpha and gamma) production by fresh and cryopreserved human mononuclear cells. Brief report

A comparative study of interferon (IFN) production (type-alpha and gamma) was carried out using Ficoll-hypaque purified fresh and cryopreserved mononuclear cells from eight normal healthy individuals. Newcastle disease virus-NDV (R2B strain) was used as an inducer for type-alpha and Staphylococcal enterotoxin-A-(SEA) for type-gamma IFN production. There was no significant difference between the titres of type-alpha and gamma-IFN and lymphocyte subpopulations of fresh and cryopreserved mononuclear cells studied under identical conditions.