Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Basement membrane: structure function relationships.

N A Kefalides

    Renal Physiology
    |January 1, 1981
    PubMed
    Summary

    Basement membranes provide filtration and elastic support through their macromolecular organization. Specific proteins and proteoglycans within the glomerular capillary basement membrane dictate its selective filtration and elasticity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A peptide of the alpha 3(IV) chain of type IV collagen modulates stimulated neutrophil function via activation of cAMP-dependent protein kinase and Ser/Thr protein phosphatase.

    Cellular signalling·2000
    Same author

    Inhibition of tumor cell proliferation by type IV collagen requires increased levels of cAMP.

    Connective tissue research·2000
    Same author

    Integrin alpha2beta1 recognizes laminin-2 and induces C-erb B2 tyrosine phosphorylation in metastatic human melanoma cells.

    Connective tissue research·2000
    Same author

    Tyrosine kinase activation in response to fungal spores is primarily dependent on endogenous reactive oxygen production in macrophages.

    The Journal of biological chemistry·2000
    Same author

    Suppression of tumor cell growth by type IV collagen and a peptide from the NC1 domain of the alpha 3(IV) chain.

    Medicina·2000
    Same author

    Regulation of tumor cell chemotaxis by type IV collagen is mediated by a Ca(2+)-dependent mechanism requiring CD47 and the integrin alpha(V)beta(3).

    The Journal of biological chemistry·2000

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Histology

    Background:

    • Basement membranes are crucial extracellular matrix structures in glomerular capillaries.
    • They consist of laminae rarae and lamina densa, housing various macromolecules.
    • These include procollagen, noncollagen glycoproteins, and proteoglycans.

    Purpose of the Study:

    • To elucidate the relationship between macromolecular composition and basement membrane function.
    • To understand how molecular organization influences filtration and elastic properties.

    Main Methods:

    • Analysis of macromolecular composition within the glomerular capillary basement membrane.
    • Investigation of the spatial organization of proteins and glycoproteins.

    Main Results:

    • Procollagen and noncollagen glycoproteins are found in both laminae rarae and lamina densa.
    • Proteoglycans are specifically located in the laminae rarae.
    • Macromolecular organization, particularly procollagen persistence, governs fibril structure and selective filtration.
    • Noncollagen extension peptides and glycoproteins contribute to elasticity.
    • Anionic nature is primarily due to acidic amino acids, with minor contributions from sialic acid and proteoglycans.

    Conclusions:

    • The specific arrangement of macromolecules within the basement membrane is key to its functions.
    • Molecular composition directly impacts the filtration selectivity and elastic resilience of the glomerular basement membrane.

    Related Experiment Videos