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A rapid centrifugation method for the isolation of polymorphonuclear leucocytes from human blood
Acta Physiologica Academiae Scientiarum Hungaricae
|January 1, 1981
Abstract:
A rapid one-step gradient centrifugation method to prepare PMN leucocytes has been worked out by which a 98--99% pure, washed granulocyte suspension of 98% viability can be obtained in 30--40 minutes. The cells prepared by this method displayed higher NBT reduction upon ingesting the same soluble DNA-anti DNA complexes than those prepared by the dextran sedimentation method.