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Prostaglandin-generating factor of anaphylaxis. Identification and isolation
The Journal of Biological Chemistry
|December 25, 1981
Summary
Researchers identified a novel mediator, prostaglandin-generating factor of anaphylaxis (PGF-A), in human lung tissue. This factor is responsible for prostaglandin formation during anaphylaxis, a key process in allergic reactions.
Area of Science:
- Immunology
- Biochemistry
- Allergy Research
Background:
- Anaphylaxis involves prostaglandin (PG) formation in human lung tissue.
- Identifying mediators of PG generation is crucial for understanding allergic reactions.
Purpose of the Study:
- To identify and characterize factors responsible for prostaglandin generation during human lung anaphylaxis.
- To investigate the role of these factors in allergic responses.
Main Methods:
- Reversed anaphylaxis of human lung tissue followed by supernatant analysis.
- Ultramembrane filtration, ethanol/chloroform solubility tests, and paper chromatography.
- Gel filtration (Sephadex G-50/G-25), CM52 and DE52 ion-exchange chromatography, and amino acid analysis.
Main Results:
- A prostaglandin-generating factor of anaphylaxis (PGF-A) was identified with a molecular weight between 500 and 5000 Da.
- PGF-A activity was associated with a ninhydrin-positive spot and had a molecular weight of 1200-1800 Da upon gel filtration.
- Amino acid analysis indicated a molecular weight of approximately 1450 Da for PGF-A, with a specific amino acid composition (Glu/Asp/Gly/Ser/Thr in a 6:3:2:1:1 ratio).
Conclusions:
- PGF-A is a novel mediator involved in human lung anaphylaxis.
- PGF-A contributes to prostaglandin generation during allergic reactions.